Journal: Infection and Immunity

Article Title: Cj1136 Is Required for Lipooligosaccharide Biosynthesis, Hyperinvasion, and Chick Colonization by Campylobacter jejuni

doi: 10.1128/IAI.00151-12

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Culture media were also supplemented with antibiotics, including ampicillin (100 μg ml −1 ), kanamycin (50 μg ml −1 ), and chloramphenicol (20 μg ml −1 ), where appropriate. table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description Source or reference Strains C. jejuni 01/51 Hyperinvasive wild-type strain 01/51 11 01/51 Δ cj1136 cj1136 mutation generated in strain 01/51 This study 01/51 Δ cj1136 :: cj1136 cj1136 mutation complemented by wild-type copy of the gene in strain 01/51 This study 81116 Δ( flaA flaB ) Double mutation in flaA and flaB genes in strain 81116 52 E. coli TOP10F′ F′ lacI q Tn10(Tet r ) mcrA Δ( mrr-hsdRMS-mcrBC ) φ80 lacZ ΔM15 Δ lacX74 recA1 araD139 Δ( ara-leu ) 7697 galU galK rpsL endA1 nupG Invitrogen Plasmids pGEM-T Easy Cloning vector encoding resistance to ampicillin Promega pGEM:1136::kan pGEM-T Easy vector carrying 1.3-kb fragment of cj1136 interrupted by Kan r cassette This study pAJ20 2.3-kb DNA fragment flanking the intergenic region between cj0652 and cj0653c with additional BamHI restriction site in the intergenic region, cloned into pGEM-T Easy vector This study pAJ22 Chloramphenicol resistance cassette cloned into pAJ20 This study pAJ22-1136 Wild-type copy of cj1136 with its own promoter cloned into pAJ22 vector This study pMA24 Source of kanamycin resistance cassette 1 pUOA18 Source of chloramphenicol resistance cassette 51 Open in a separate window Bacterial strains and plasmids

Techniques: Plasmid Preparation, Mutagenesis, Generated, Cloning, Clone Assay